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Nucleic acid detection test strips (line elimination method)

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Nucleic acid detection test strips (line elimination method)

The CRISPR product detection strip applies the principle of sandwich immunochromatography. The specific FITC monoclonal antibody is labeled on the colloidal gold, and streptavidin (SA) is immobilized on the detection line (T line) of the NC membrane. The secondary antibody is immobilized on the quality control line (line C) of the membrane. Used to detect whether the target probe is cleaved.
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Nucleic acid detection test strips (line elimination method)

【Product number】

  C008

【product description】

      The CRISPR product detection strip applies the principle of sandwich immunochromatography. The specific FITC monoclonal antibody is labeled on the colloidal gold, and streptavidin (SA) is immobilized on the detection line (T line) of the NC membrane. The secondary antibody is immobilized on the quality control line (line C) of the membrane. When there is a certain amount of FAM-biotin (or FITC-biotin) double-labeled probe in the sample, it forms a polymer of colloidal gold-FITC monoclonal antibody-FAM-biotin with colloidal gold. Capture, so that the T line develops color, and the C line captures the colloidal gold labeled with FITC monoclonal antibody and develops color; when the target molecule exists, the Cas protein is activated and non-specific ssDNA cleavage occurs, the dual-labeled probe is degraded, and the colloidal gold-FITC If the polymer of mAb-FAM cannot be captured by the T line, the T line will not develop color, and the C line can still capture the colloidal gold labeled with FITC mAb and develop color.

      Intended use: used to detect whether the target probe is cleaved

【Storage conditions】

  Store in a dry place at 2°C~30°C away from light


【Product ordering information】

name article number Specification Remark
Nucleic acid detection test strips (line elimination method) C008 50T Compatibility: FAM-biotin (or FITC-biotin) dual-labeled probe

 

Keyword:
核酸试纸条
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