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Cas14a1 protein

Cas14a1 nuclease is an endonuclease that specifically binds and cleaves target ssDNA under the guidance of tracrRNA:crRNA (or sgRNA) without the need for a PAM site. Compared with other Cas proteins, the molecular weight of Cas14 protein is generally smaller (400-700aa); similar to Cas12, Cas14a1 can also bind target nucleic acid and activate its ssDNA trans-cleavage activity, so it can be used for molecular detection of target nucleic acid; Unlike Cas12, Cas14a1 can only bind to ssDNA targets, so the amplified and enriched target nucleic acid needs to be treated with T7 exonuclease, and one of the amplification primers needs to be modified with phosphorothioate to ensure T7 exonuclease Dicer cleaves only one of the strands, leaving the ssDNA target strand for Cas14a1-mediated molecular detection.
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C015
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Cas14a
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Cas14a1 protein

【Product number】

  C015

【product description】

      Cas14a1 nuclease is an endonuclease that specifically binds and cleaves target ssDNA under the guidance of tracrRNA:crRNA (or sgRNA) without the need for a PAM site. Compared with other Cas proteins, the molecular weight of Cas14 protein is generally smaller (400-700aa); similar to Cas12, Cas14a1 can also bind target nucleic acid and activate its ssDNA trans-cleavage activity, so it can be used for molecular detection of target nucleic acid; Unlike Cas12, Cas14a1 can only bind to ssDNA targets, so the amplified and enriched target nucleic acid needs to be treated with T7 exonuclease, and one of the amplification primers needs to be modified with phosphorothioate to ensure T7 exonuclease Dicer cleaves only one of the strands, leaving the ssDNA target strand for Cas14a1-mediated molecular detection.

【Storage conditions】

-20℃ sealed storage

【Remark】

♦  This product is recombinant protein

♦  The design and stability of sgRNA have a great influence on the reaction system. Before the reaction, whether the sgRNA is degraded can be detected by gel electrophoresis.


【Product ordering information】

name article number Specification (Concentration) Remark
Cas14a1 protein C015S 100pmol(10μM) Contains 50% glycerin
C015M 1000pmol(10μM) Contains 50% glycerin

 

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Cas14a
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