T7 Endonclease I

T7 Endonclease I

【product overview】

T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double stranded DNA. The cleavage site is the first, second or third phosphodiester bond that is 5´ to the mismatch.

【Catalog#】
C007

【Introduction】

Magigen T7 Endonuclease can be used to detect gene mutation, SNP, TALEN or CRISPR / cas9 mutants, identify mismatched DNA, and resolve four-way cross DNA or branched DNA. Detection of heteroduplex or cleavage of DNA; It is used to randomly cut linear DNA for shot gun cloning, etc.

【Unit Definition】

The amount of enzyme required to convert pUC (AT)* of a 90% or more superhelier cross-shaped structure into a linear structure of more than 90% in a 50 μl reaction system at 37°C for 1 hour is defined as one unit of activity.

【Product Features】

T7 Endonuclease I is a substrate structure-selective enzyme that exhibits different cleavage activities for different DNA substrates. Therefore, when cutting a specific substrate, it is necessary to explore and control the amount of enzyme and reaction time.

【Storage Conditions and Expiration】

Sealed at -20℃

►【Ordering Information】