CRISPR Cas12b protein
Magigen CRISPR Cas12b can be used to detect DNA templates and amplify signals by designing ssDNA probes labeled with fluorescent groups or other markers at both ends. Common detection methods include real-time fluorescence and colloidal gold chromatography. The Cas12b also react more active in envirnment that has higher tempeture (45 ~ 65℃). Hence, this system has been widely used in gene editing of micro organisms, plants and animals, and has great practical application value in molecular diagnosis.
Magigen AapCas12b protein, derived from Alicyclobacillus acidiphilus, is an RNA-mediated endonuclease that binds to and cleaves specific sites of target DNA under the guidance of single-stranded RNA. While Cas12b recognizes and cleaves target double-stranded DNA under the guidance of guide RNA, its collateral cleavage activity is activated, which can efficiently cleave non-specific single-stranded DNA (ssDNA). CRISPR/Cas12b can be used to detect DNA templates and amplify signals by designing ssDNA probes labeled with fluorescent groups or other markers at both ends.
♦ This product is a recombinant protein
♦ This product 1pmol=132 ng.
♦ The design and stabilization of sgRNAs have a great influence on the reaction system, and whether sgRNAs can be degraded by gel electrophoresis before the reaction is carried out.
Cas12b designed to be using in molecular detection for detecting DNA. If the dectecting target RNA needs to combine the DNA enzyme originated from itself, methods like RPA, LAMP and PCR are applying to Cas12b as well.
【Storage Conditions and Expiration】
Sealed at -20℃
According to tested results below, using different concentrations of Cas12a protein to cut ssDNA, it can be found that a sufficient amount of Cas12b can cut DNA in about 10 minutes.
|CRISPR Cas12b protein||C006S||100pmol（2μM）||50% Glycerine contained|
|C006M||1000pmol（10μM）||50% Glycerine contained|
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