CRISPR Cas12a protein 

CRISPR Cas12a protein

Magigen CRISPR Cas12a protein is a programmable DNA endonuclease guided by a single guide RNA (gRNA). Cas12 requires only a single crRNA, simple designed. It is more easier to delivered into cells with less volume. Cas 12 provides new target sequence selection with TTTN PAM, suitable for a broad reaction temperature range of 20℃-48℃.

【Catalog#】

  C001

【Introduction】

With its pure protein system that resists distraction and low off-target rate, The CRISPR-Cas9 technologies is a safe and common method in the field of gene editing.  

【Unit Definition】

♦ This product is a recombinant protein that carries NLS fragments at both the N- and C-terminals.

♦ This product 1pmol=151 ng.
♦ The design and stabilization of sgRNAs have a great influence on the reaction system, and whether sgRNAs can be degraded by gel electrophoresis before the reaction is carried out.

【Product Features】

1. Requires only a single crRNA, easy design. It is more easily delivered into cells with less volume.
2. Provide new target sequence selection with TTTN PAM.
3. a broad reaction temperature range of 20℃-48℃.
4. The affiliated cleavage activity generated after cleavage of the target site can be used in a highly sensitive and specific nucleic acid detection scheme.

【Storage Conditions and Expiration】

Sealed at -20℃

【Product Performances】

According to tested results below, using different concentrations of Cas12a protein to cut ssDNA, it can be found that a sufficient amount of Cas12a can cut DNA in about 12 minutes. 

►【Ordering Information】